Single-cell analysis in CAR T cell therapy

Scailyte leverages the power of single-cell technologies and its proprietary data analysis platform ScaiVision. This union enables single-cell level precision in R&D and development of novel targeted assays in manufacturing of cell therapies. While cutting production and treatment costs of up to 50%, Scailyte’s solutions for patient stratification, optimised R&D and QA processes will make cell therapy accessible to more patients with maximum treatment efficacy.

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ScaiVision AI platform: project workflow to facilitate drug development

ScaiVision unravels hidden secrets of complex single-cell multiomics data to extract composite biomarkers associated with different cell populations. Using a convolutional neural network and representation learning, ScaiVision automatically learns molecular patterns associated with relevant clinical outcomes. These signatures can then be applied to classify new samples and develop diagnostic assay prototypes.

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Discovering next-generation biomarkers through integration of single-cell biology and artificial intelligence

Single-cell technologies have enabled the generation of vast amounts of data from human tissues with unprecedented resolution. While numerous cell atlas efforts have made strides toward describing and cataloging cellular complexity, the full potential of single-cell data in biomarker discovery and clinical applications has yet to be unlocked, in part due to the challenges in analyzing such high-dimensional, complex data. Scailyte has developed a novel approach combining single-cell analysis, integration of clinical data, and a tailor-made supervised machine learning platform, which allows for targeted and sensitive biomarker discovery. Here we highlight several discovery projects across different disease areas, including oncology and cell therapy.

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Identification of ALP+/CD73+ defining markers for enhanced osteogenic potential in human adipose-derived mesenchymal stromal cells by mass cytometry.

Adipose-derived mesenchymal stem cells represent a relatively novel cell therapeutic modality for promoting bone healing, however they remain relatively uncharacterized on a subpopulation level. Using the forefather algorithm of ScaiVision, CellCNN, the authors were able to exploit CyTOF data to identify an ALP and CD73-double positive population as having a high bone differentiation potential. They could also show that these cells could not only be used to identify promising cell lines for use in bone regeneration, but their levels could also be used as a quality control during manufacturing.

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High-dimensional single-cell analysis predicts response to anti-PD-1 immunotherapy

Although it has driven a paradigm shift in how cancer is treated, targeted immunotherapy is oftentimes plagued by low response rates, highlighting the urgent need for biomarkers predictive of response. Here, CellCNN was used to further characterize and confirm the identity of a specific subpopulation of monocytes as being strongly associated with response to anti-PD-1 immunotherapy in stage IV melanoma, paving the road for the selection of tailored treatments for patients.

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